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Poster Presentation 6-38
The Biotechnological Aspects of Transformation of Polysaccharide-containing Vegetable Raw Materials
Victoria Aizenberg, Victoria Pitchko, Natalia Gregyrchak, Irina Tverdokhlib, Serghei Surchin, Rodion Kartavykh, Nadezhda Sosnina, Boris Berenshtein, Lidia Moskalenko
Institute of Microbiology and Virology, National Academy of Sciences of Ukraine (IMV NASU) 154 Zabolotny St., 03143, Kyiv, Ukraine
Telephone: 38044 266-99-36; Fax: 38044-266-23-79; E-mail: victorsbz@narod.ru
The high adaptability of Micromycetes, which allows them to survive in varying environmental conditions, is determined by their ability to induce synthesis of various enzymes.
Research carried out at the Department of Physiology and Systemization of Micromycetes of the IMV NASU is concentrated on developing microbial strains for overproducing a variety of enzymes (cellulases, pectinases, lipases, inulinases, etc.) on the basis of collected cultures of non-toxic strains of fungi with further use in biotechnology and for direct utilization of the obtained enzymes in bio-conversion of phyto-material into costly foodstuffs and forage products, etc.
A collection of Micromycetes including as many as 8000 fungal cultures, exceeding 400 species of 105 genera, is maintained at the Department. The Department has at its disposal a prospective producer of microbial protein – Thielavia Sp., having high cellulolytic activity. The fungus actively transforms difficult to access cellulose substrates, sawdust, straw, beet cake, etc. with biomass turnout of up to 15-20 g/L and more; protein content in such bio-mass is about 40%. Dried biomass of the fungus may be used as feed additive to rations of pigs, chickens, etc.
Penicillium was selected from strains of active cultures belonging to the genera Thielavia rhizopus for production of cellulolytic, pectolytic, and lipolytic enzymes,. The strains Rhizopus cohnii, an overproducer of exolipase; and Penicillium dierckxii 57699, an overproducer of pectinesterase, were also found. Lipase from Rhizopus cohnii, which can transform lipid-containing raw materials, will be used to produce modified fats, as well as unsaturated fatty acids.
Pectinesterase from Penicillium dierckxii utilizes pectin-containing raw materials and can be used to obtain low-degree methoxylated pectins and to prepare enzymatically modified pectin substances that could be used as potential therapeutic agents.
At present our group carries out screening of strains of fungal origin with the increased ability to synthesize extra-cellular inulinase for practical applications.
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