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Poster Presentation 6-11
Nitrogen Source Effect on the Invertase Production by Saccharomyces cerevisae
L. P. Rodríguez-Pascual, V. Rivera-Martínez., R. B. Ávalos-Fernández., O. García-Kirchner
Departamentos de Biología y Bioprocesos Unidad Profesional Interdisciplinaria de Biotecnología Instituto Politécnico Nacional. (UPIBI-IPN), Av. Acueducto S/N, Barrio La- Laguna, Ticomán. México, 07340, D.F., MÉXICO
Telephone/Fax: (55) 5729-6000; E-mail: ogarcia@acei.upibi.ipn.mx
The primary application of invertase ( b-D-fructofuranoside fructohidrolase, E.C. 3.2.1.26) from Saccharomyces cerevisiae is in the confectionary industry. In Mexico, much of this enzyme is imported. The influence of the carbon source during invertase production is well known, but little is understood about the nitrogen source influence.
We examine three different nitrogen sources using three Saccharomyces cerevisiae varieties for invertase production. All of the strains tested benefited in terms of invertase production when CSL was used; however, this effect was not observed when the specific rate growth was considered. The strain with highest production reached similar levels of enzyme using both CSL and (NH4)2SO4 as nitrogen sources.
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