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Poster Presentation 3-55
Production of Erythritol by an Osmophilic Mutant of Candida magnoliae in Fed-batch Culture
Eun-Sung Koh1, Do-Yup Lee1, Kyu-Ho Choi, Yeon-Woo Ryu2, Jung-Kul Lee3 and Jin-Ho Seo1
1Department of Food Science and Technology Seoul National University, Suwon , 441-744, Korea
2Department of Molecular Science and Technology Aju university, Suwon 442-749, Korea
3Bolak Company Limited Kyonggi-Do, 307-1, Korea
Telephone: 82-31-290-2591; Fax: 82-31-294-1336; E-mail:
An osmophilic mutant of Candida magnoliae was used to produce erythritol from glucose with high yield and productivity. The mutant produced erythritol in a batch culture containing 20% (w/v) glucose medium with 0.54 g/L/hr of erythritol productivity and 43% of conversion yield based on glucose. The fermentation preparations were further improved by adopting a two-stage fed-batch process where the first stage was operated for cell growth and the second stage for erythritol production.
When the initial glucose concentration was adjusted to 400 g/L in the second stage, 2.8 g/L/hr of overall erythritol productivity and 41% of conversion yield were achieved.
A high glucose concentration in the production phase resulted in formation of organic acids such as gluconic acid and butyrate
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