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Poster Presentation 3-44
Production of Arachidonic Acid in a Fed-batch System by M. alpina DSA-12
Byung-Hee Hwang, chang-Yeol Park and Yeon-woo Ryu
Department of Molecular Science and Technology Ajou University Suwon 442-749 Korea
Telephone: 82-2-31-219-2455; Fax: 82-2-31-216-8777; E-mail: sparrow93@hanmail.net
Arachidonic acid (all-cis-5, 8, 11, 14-eicosatetranoic acid, ARA) is a polyunsaturated fatty acid (PUFA) containing 20 carbon atoms with four double bonds. The family of ω-6 PUFA including ARA as well as δ-lineolic acid (GLA) was served as intermediates in the formation of several key prostaglandins and leukotrienes. We conducted a large-scale batch and fed-batch culture of Motierella alpina DSA-12, a hyper-producing strain of ARA in a growth associated manner, for the purpose of high-level production of Arachidonic acid. A batch culture (2.5/L) was carried out to produce ARA, the medium containing 5% glucose, 1.8% corn steep powder (CSP) and 50˚C;100 mg/L MnSO4·7H2O under 25˚C; and 0.5 vvm without pH control. As a result, we obtained a high concentration of biomass (20.5 g/L) and ARA (3.9 g/L). The fed-batch culture was performed in an initial medium containing 2% glucose and 1% CSP; ammonia solution (7, 14 and 28%) sodium hydroxide (0.5 N) were prepared to control the pH level in the period of cultivation. The fed-batch study showed a relatively high productivity of cell mass (70.5 g/L) and ARA content (18.3 g/L) when 14% ammonia solution was alternatively used to control the pH in the cultivation period. Therefore, when compared to batch culture, it was strongly suggested that the high content of ARA could be obtained by fed-batch culture using Motierella alpina DSA-12. These results implied that the fed-batch culture of Motierella alpina DSA-12 was feasible for industrial purposes and could be used in the commercial production of ARA.
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