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Poster Presentation 3-43
Fumaric Acid Production by Rhizopus oryzae KCTC 6946
Jin-Nam Kim, Young-Jung Wee, Jong-Sun Yun and Hwa-Won Ryu
Faculty of Chemical EngineeringChonnam National UniversityGwangju 500-757, South Korea
Telephone: +82-62-530-1842; Fax: +82-62-530-1849; E-mail: hwryu@chonnam.ac.kr
Biological production of organic acids has attracted great interest as an alternative to chemical production because of environmental pollution and exhaustion of petroleum feedstocks. As fumaric acid possesses a double bond and two carboxylic groups, it is an effective intermediate in chemical synthesis involving esterification and polymerization reactions. It is not only a good source for sizing resins in the paper industry but also acidulant in the food and pharmaceutical industry. In particular, fumaric acid is used as a hydrogen acceptor in bioconversion into succinic acid.
In this study, fumaric acid production by Rhizopus oryzae KCTC 6946 using flask and rotary biofilm contactors was investigated. As fumaric acid production in Rhizopus species is non-growth associated, the effects of carbon and nitrogen sources on fumaric acid production were studied in flask culture. The optimum weight ratio of carbon and nitrogen source (g-starch/g-CSL) was 41.7. The yield and volumetric productivity of fumaric acid were 21.7% and 0.097 g/L/h, respectively, at 35 and pH 5.5. Based on these results, fumaric acid fermentation was carried out with rotary biofilm contactor that was constructed of 3.5-L fermenter with 1.0-L working volume. The yield and volumetric productivity of fumaric acid in rotary biofilm contactor were 33.8% and 0.595 g/L/h, respectively. These results were 1.54 and 6.13 fold higher than those of flask culture.
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