Poster Presentation 3-34
Using Rice Waste as the Substrate in Fermentation Processes to Obtain Useful Products
A. M. Blosfeld1, Filho A. Castro2 and R. Wendhausen Jr. 3
1Coord. Biotechnology Lab. (IPT/FURB), University of Blumenau Rua Araçatuba 83, Blumenau,Santa Catarina 89030-080 Brazil
2Undergraduate Student, Chem. Eng. (FURB)
3Prof. Chemical Dept. (FURB), Universidade Regional de Blumenau, Lab. Biotecnologia – IPT/FURB, Rua Araçatuba, 83. 89030-080 - Blumenau – SC
Telephone/Fax: + 55 47 323 7200; E-mail: ana@furb.br
One of the major problems and biggest challenges of this new century is the lack of food supply. It is important to consider alternative ways to obtain food; in particular, single cell proteins as a food supply. There are enormous quantities of agricultural waste discarded, such as skin, bran and wastewater from the rice industry. This research studied the production of total extracellular protein in a fermentation process using rice waste as a substrate. All the experiments were run in a shaker, using different compositions of substrate, such as: synthetic medium with 10% rice skin added; synthetic medium with 10% rice bran added; rice waste water with 10% rice skin added; and rice waste water with 10% rice bran added. Two microorganism strains were used, Aspergillus niger and Mucor rouxii, in all media. The temperature, pH, air and agitation were previously established and kept constant. Biomass concentration was determined by dry weight. The total extracellular protein was measured spectrophotometrically with Bradford Coomassie Blue (modified in the Bio-Rad Laboratory). Results showed that it was possible to obtain and enhance the biomass and total extracellular protein production using rice waste as a substrate. A higher biomass production (18.4 fold) and a higher total extracellular protein production (6.9 fold) were obtained when 10% of the rice bran was added to the synthetic medium using Mucor rouxii. For Aspergillus niger the best biomass enhancements were achieved when 10% of the rice bran was added to the synthetic medium (12.34 fold); and for total extracellular protein production when rice waste water with 10% rice bran added was used as a substrate (4.6 fold). Additionally, the maximum yield of biomass and total extracellular protein was achieved after 48-hour fermentation in all runs.
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