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Poster Presentation 3-32
Solubilization of Insoluble Phosphate Salts by Immobilized Microorganism
Won-Bong Lim1, Don-Hee Park1,2,3, Il Jung1, Ki-Young Lee1, Choon-Hyoung Kang1, Sung-Hyo Chough1, Chung-Han Yoon4, Ro-Dong Park5 and Si-Wouk Kim6
1Faculty of Chemical Engineering 2Institute of Bioindustrial Technology 3 Research Institute of Catalysis 4Dept. of Mineral and Energy Resources Engineering 5Dept. of Agricultural Chemistry, Chonnam National University, Kwangju, 500-757, KOREA
6Dept. of Environmental Engineering, Chosun University, Kwangju 501-759, KOREA
Telephone: 82-62-530-1841; Fax: 82-62-530-1849; E-mail: dhpark@chonnam.ac.kr
This research examined the solubilization efficiency of insoluble phosphate salts by Pantoea agglomerans, known as inorganic phosphate solubilizing microorganisms, and examined continuous phosphate reproduction using immobilized P. agglomerans on calcium alginate beads as supporters. In the HY medium during P. agglomerans cultivation, 465 mg/L of phosphate was solubilized for 48 hours. When insoluble rock phosphate salts were used, phosphate was 86.09 mg/L in broth.
When immobilized P. agglomerans was used, 740 mg/L of phosphate was solubilized in the HY medium during 120 hours; and 182 mg/L of phosphate was solubilized when rock phosphate was added instead of hydroxyapatite. In the case of culture of P. agglomerans on constant medium pH, phosphate was produced 357 mg/L after 18 hours. And in the case of culture of immobilzed P. agglomerans bead, phosphate was produced maximum 295.6 mg/L after 120 hours. Also, when using rock phosphate as insoluble phosphate salts, phosphate was respectably produced 190.3 and 195.2 mg/L after 36 hours at free cells and immobilized cells.
These experimental results show the possibilities for biofertilizers, which convert insoluble inorganic phosphate salts to plant-usable phosphates by immobilized using microorganisms with calcium alginate.
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