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Poster Presentation 2-28
Cassava Flour Wastewater as Substrate for Biosurfactant Production
M. Nitschke and G. M. Pastore
Faculdade de Engenharia de Alimentos , Departamento de Ciência de Alimentos - UNICAMP - CP 6121, CEP: 13083-970, Campinas, SP – Brasil
E-mail: nitschke@bol.com.br
Biomolecules that show surface-active properties are classified as biosurfactants. Compared to synthetic compounds, biosurfactants offer the advantages of little or no environmental impact and the possibility of in situ production. However, from an economic standpoint, biosurfactants are not yet competitive with synthetics. The use of agro-industrial byproducts could be explored to reduce production costs. In this work the production of biosurfactants by Bacillus sp. LB5a using cassava flour wastewater (manipueira) as a substrate was evaluated. Five manipueira-based media were prepared and tested. An aliquot of 1 mL of inoculum was transferred to 15 mL of each manipueira-based medium contained in 50 mL Erlenmeyer’s flasks, and incubated for 72 h, 150 rpm at 30°C. The broth was centrifuged at 10.000 rpm for 15 min and the supernatant was submitted to superficial tension (ST) and critical micelle dilution (CMD) measurements. All medium tested showed a significant decrease in ST. Manipueira medium with no solids showed the best results: 26.38 to ST; 26.8 to CMD-1 and 33.96 mN/m to CMD-2; the CMC-1 of this broth was 114 indicating a higher biosurfactant concentration. These results demonstrate that manipueira has great potential as a substrate for biosurfactant production.
Acknowledgements: authors’ thanks to CAPES and FAPESP for financial support.
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