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Poster Presentation 1-18
Selectivity of the Degradation of Sugarcane Bagasse by the White-rot Fungus Ceriporiopsis submermispora
Sirlene M. Costa1, Adilson R. Gonçalves1 and Elisa Esposito2
1Depto. Biotecnologia – FAENQUIL, CP 116, CEP 12.600-000 Lorena, SP, Brazil
2Núcleo Integrado de Biotecnologia, Universidade de Mogi das Cruzes, Brazil
Telephone: 55-12-553-3422, ext. 235; Fax: 55-12-553-3165; E-mail: adilson@debiq.faenquil.br
In the present work sugarcane bagasse was degraded by the white-rot fungus Ceriporiopsis subvermispora and submitted to chemical analysis, where the loss of mass and components were determined. The decayed bagasse was treated with 5 mL H2SO4 72 %; after 7 min stirring at 45oC, 25 mL of water were added to the mixture, which was post-hydrolyzed under 1.05 bar for 30 min. The insoluble portion (Klason lignin) was quantified and soluble lignin determined by absorption at 280 nm from alkaline solutions; sugars were quantified by HPLC. The enzymatic activities of lignin peroxidase (LiP), manganese peroxidase (MnP), laccase (Lac) and xylanase (Xyl) were determined after extraction with 800 mL of solution containing 50 mM sodium acetate buffer pH 5.5 and 0.01 % Tween 80. The solid state fermentation of 100 g of bagasse was carried out in bioreactors, with and without additional salt solution for 15, 30 and 45 days. The results showed that the major selectivity for degradation of lignin and preservation of carbohydrates was obtained in the bioreactor with 45 days (2% of inoculum) and without salt solution. A higher loss of mass was obtained with 30 days (1% of inoculum). On the other hand, the values of enzymatic activities of xylanase produced by Ceriporiopsis subvermispora in sugarcane bagasse were high and also the loss of glucan was high. Enzymatic activities of Lac and LiP were not detected.
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