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Oral
Presentation 7-02 Phenylboronate-Chitosan Resins for Adsorption of Beta-amylase from Soybean Extracts E.J.
Arruda1 and C.C. Santana2 1Pharmacy and Biochemistry Catholic University Dom Bosco, UCBD Campo Grande, MS Brazil C.P 100, CEP 79002-250
2School of Chemical Engineering DPB, UNICAMP Campinas, SP Brazil C.P. 6066, CEP
13083-970 Telephone: (55) 19-37883917; Fax: (55) 19-37883918; Email: santana@feq.unicamp.br
Isolation and purification of bioproducts
starting from crude extracts can be obtained by methods based on affinity for a
certain molecule that binds specifically and reversibly to a ligand molecule
immobilized in a porous matrix. The
b-amylase (b-1,4-glucan maltohydrolase,
E.C 3.2.1.2) is an exo-enzyme that removes units of maltose of non-reduced
terminals of the polysaccharides chains.
There is great industrial interest for the enzyme in the production of
rich syrups in maltose, and interest for medical use as an intensifier of the
fagocitose in carcinomas. Presented
here are the kinetic curves and the isotherms of adsorption of the crude
extracts, as well as the breakthrough curves for a frontal chromatographic
separation method of a commercial sample of beta-amylase from soybean in the
interaction with the adsorbents prepared starting from a chitosan matrix with
3-aminophenylboronic acid. Those
results were compared to similar data obtained with an agarose based commercial
gel (Matrex gel PBA 30 - Amicon, USA).
The adsorbent’s behavior was markedly different from that of phenylboronate-chitosan
(PBC), to which the enzyme was rapidly adsorbed and which required much more
drastic elution conditions. Keywords: purification; beta-amylase;.soybean;
phenyl-boronate; chitosan.
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