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Oral Presentation 6-07 Investigation of Cellulase and Hemicellulase Production in Penicillium
brasilianum using Capillary
Electrophoresis
Henning Jørgensen,
Lisbeth Olsson
Center for Process Biotechnology BioCentrum-DTU Building 223 Technical University of Denmark DK-2800 Kgs. Lyngby Telephone: (45) 45252678; Fax: (45) 45884148; E-mail: henning.Jorgensen@biocentrum.dtu.dk
There
is a growing demand for specific, efficient and cheap cellulases and
hemicellulases for use in formulation of washing powders, in textile
production, in the pulp and paper industries and for degradation of
lignocellulosic material in the production of bio-ethanol. Many filamentous fungi are good enzyme
producers, such as Trichoderma, Aspergillus and Penicillium, but
many details about the regulation of enzyme production remain unknown. It is therefore of interest to gain more
information about production of the many different cellulases and
hemicellulases and their regulation during growth on different carbon sources.
The
enzymatic assays used for measuring production of the enzymes are time
consuming, not always specific, and do not give any information about the
presence of iso-enzymes. Capillary
electrophoresis is an analytical technique capable of separating and measuring
the proteins present in the cultivation broth.
It is fast and offers automatic analysis of a large number of samples.
Unlike enzymatic assays, capillary electrophoresis makes it possible to measure
several different enzymes with similar activity, which results in more detailed
information about changes in the enzyme production pattern during growth on
complex substrates.
Capillary electrophoresis analysis, in association with traditional enzymatic assays, has been used to study the production of cellulases and hemicellulases in the filamentous fungi Penicillium brasilianum (IBT 20888) during growth on different carbon sources (glucose, xylose, cellulose and xylan) – aiming at identifying the regulation of the enzymes.
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