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Oral
Presentation 6-03 Xylanase Production by Penicillium canescens in Solid-State Fermentation on
Agricultural Wastes Y.
Bakri, P. Jacques, and P. Thonart Centre Wallon de Biologie Industrielle Agricultural University of Gembloux Passage des Deportes 2 Gembloux, 5030 Belgium Telephone: +3281622311; Fax: +3281614222; E-mail: jacques.p@fsagx.ac.be
The use of purified xylan enhances the cost of
enzyme production and is a major limitation to the economic feasibility of
bioconversion and utilization of lignocellulosic materials. Therefore, production of xylanase by Penicillium
canescens 10-10c under solid state culture (SSC) was tested with five
easily available substrates as carbon sources:
wheat straw, wheat bran, sugar beet pulp, soybean meal, and corn
cob. The effect of different nitrogen
sources on xylanase production was also investigated. A high xylanase activity was obtained by using wheat straw as a
carbon source and yeast extract as a nitrogen source. The optimal initial ratio of carbon source to mineral solution
for xylanase production was 1:5. A
particle size of wheat straw <1 mm produced a higher enzyme activity. Under these conditions, yields as high as
10164 U/g of carbon source were obtained.
Xylanase production was improved by the addition of 0.4 g xylan to the
wheat straw. Xylanase activity also
increased in the presence of 2% (w/w) xylose and glucose.
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