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Oral
Presentation 2-02 Saccharification of Marine Algal Starch Using Marine Bacteria Mitsufumi Matsumoto3, Takuya Koizumi1, Nobukazu Suzuki2 Hiroshi Ohata3, Hideaki iyashita1, Tadashi Matsunaga1
1Department of Biotechnology, Tokyo University of Agriculture and Technology 2-24-26,
Naka-cho, Koganei, Tokyo, Japan, 184-8588 2Chemical and Insulation Technology Group, Power and Industrial Systems R&D Center,
Toshiba Co. ltd. Tsurumi-ku, Yokohama, Japan, 230-0045
3New Energy and Technology Development Department, Electric Power Development, Co.
Ltd., Chuo-ku, Tokyo, Japan, 104-8165
Phone: +81-423-88-7020; Fax: +81-423-85-7713; E-mail: mitsufum@cc.tuat.ac.jp
The
photosynthetic products of marine algae such as starch or polysaccharide can be
utilized as substances for bioethanol production. For direct production of bioethanol from marine algal biomass,
halotolerant enzymes are required for saccharification. Amylase is normally used for
saccharification; however, terrestrial amylase can’t be used due to decreasing
activity under saline conditions. The purpose of this study is to
obtain marine bacteria producing amylase and evaluate a process for
saccharification of photosynthetic products from marine algae by marine
amylase.
The
first step involves screening of marine bacteria producing amylase. Marine bacteria isolated from the coastline
of Japan were tested for production of glucose as reduced sugar from potato
starch. The Pseudoalteromonas sp. NKMB 0074 was found to have the highest
glucose production rate from 191 isolates.
Saccharification of marine algal starch using amylase produced by Psuedoalteromonas sp. NKMB 0074 was also investigated. The release of reduced sugar was confirmed to incubate with Pseudoalteromonas sp. NKMB 0074 and marine algal biomass. Furthermore, fermentation using saline-adapted Saccharomyces cells was investigated.
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